We have developed an assay for the binding of [3H]-dihydrorotenone ([3H]DHR), an analogue of the pesticide rotenone, to the mitochondrial enzyme, complex I, in intact human platelets. The highly hydrophobic nature of dihydrorotenone, which diffuses easily through biological membranes, rendered the isolation of mitochondrial fractions unnecessary. This… (More)
Figures and Tables
Sorry, we couldn't extract any figures or tables for this paper.