Assay formats involving acridinium-ester-labeled DNA probes.

@article{Arnold1989AssayFI,
  title={Assay formats involving acridinium-ester-labeled DNA probes.},
  author={L. J. Arnold and P. Hammond and W. Wiese and N. C. Nelson},
  journal={Clinical chemistry},
  year={1989},
  volume={35 8},
  pages={
          1588-94
        }
}
We describe the development of several hybridization assay formats involving acridinium-ester-labeled DNA probes. The simplest of these is a homogeneous assay procedure that requires only three steps to complete, including a 5-s detection step. Using this format, we have detected target sequences in the 10(-16) to 10(-17) mol range; when rRNA is the target, this translates to 3000 to 300 bacterial organisms. The entire assay can be carried out in less than 30 min. This is the first homogeneous… Expand
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