Assay for tyrosine hydroxylation activity of tyrosinase from betalain-forming plants and cell cultures.

@article{Steiner1996AssayFT,
  title={Assay for tyrosine hydroxylation activity of tyrosinase from betalain-forming plants and cell cultures.},
  author={Ulrike Steiner and Willibald Schliemann and D ieter Strack},
  journal={Analytical biochemistry},
  year={1996},
  volume={238 1},
  pages={72-5}
}
In our studies on tyrosinase-catalyzed tyrosine hydroxylation, possibly involved in betalain biosynthesis, we have evaluated different assays for the detection and quantification of the enzymatic product Dopa with respect to sensitivity, simplicity, and suitability for automatization. A tyrosinase assay including reversed-phase high-performance liquid… CONTINUE READING