Assay for enzymatic protoporphyrinogen oxidation, a late step in heme synthesis.

Abstract

Detailed directions are given for the spectrophotometric and fluorometric assays for protoporphyrinogen oxidation in rat liver mitochondria and other tissues. Linearity of the two assays with enzyme concentration is demonstrated. Some possible assay modifications and sources of error are mentioned. The fluorometric assay appears more sensitive, while the… (More)

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Cite this paper

@article{Jacobs1982AssayFE, title={Assay for enzymatic protoporphyrinogen oxidation, a late step in heme synthesis.}, author={Nicholas J. Jacobs and Judith M. Jacobs}, journal={Enzyme}, year={1982}, volume={28 2-3}, pages={206-19} }