Assay discrepancy in mild haemophilia A

  title={Assay discrepancy in mild haemophilia A},
  author={E. Armstrong and A. Hillarp},
  journal={European Journal of Haematology},
  • E. Armstrong, A. Hillarp
  • Published 2014
  • Medicine
  • European Journal of Haematology
  • There are three main methods used to assay factor VIII (FVIII) activity: the one‐stage and two‐stage clotting assays and the two‐stage chromogenic method. The most commonly used assay for the diagnosis of haemophilia A is the automated one‐stage FVIII assay. The classical two‐stage FVIII assays are less frequently used. The chromogenic FVIII:C assay is a variant of the two‐stage assay. It is easier to use and therefore used more commonly. Recently significant assay discrepancy has been… CONTINUE READING
    12 Citations

    Topics from this paper

    Method validation and clinical utility of chromogenic factor VIII assay compared to one-stage assay
    • 4
    Discrepancies between the one‐stage clotting assay and the chromogenic assay in haemophilia B
    • 19
    Cross-reacting Material-positive Hemophilia A Diagnosed in a Patient with a Spontaneous Thigh Hemorrhage
    • PDF
    Challenges in the laboratory analyses of bleeding disorders.
    • M. Chitlur
    • Chemistry, Medicine
    • Thrombosis research
    • 2012
    • 40
    Laboratory Monitoring of Factor VIII and Factor IX in Hemophilia Patients
    • PDF
    Factor levels in carriers of haemophilia are associated with familial severity: a Danish single centre study
    • 3


    One‐stage and chromogenic FVIII:C assay discrepancy in mild haemophilia A and the relationship with the mutation and bleeding phenotype
    • 53
    Tyr346→Cys mutation results in factor VIII:C assay discrepancy and a normal bleeding phenotype – is this mild haemophilia A?
    • 33
    Discrepancy between one-stage and chromogenic factor VIII activity assay results can lead to misdiagnosis of haemophilia A phenotype.
    • 41
    • Highly Influential
    • PDF
    Prevalence, biological phenotype and genotype in moderate/mild hemophilia A with discrepancy between one‐stage and chromogenic factor VIII activity
    • 37
    Assay discrepancy in mild haemophilia A: entire population study in a National Haemophilia Centre
    • 34
    The molecular basis of hemophilia A: genotype-phenotype relationships and inhibitor development.
    • 125