Aspergillus niger-galactosidase production by yeast in a continuous high cell density reactor

@inproceedings{Domingues2004AspergillusNP,
  title={Aspergillus niger-galactosidase production by yeast in a continuous high cell density reactor},
  author={Lucı́lia Domingues and Nelson Lima and Jos{\'e} Ant{\'o}nio Couto Teixeira},
  year={2004}
}
The continuous production of extracellular heterologous -galactosidase by a recombinant flocculating Saccharomyces cerevisiae, expressing thelacA gene (coding for -galactosidase) of Aspergillus niger was investigated. A continuous operation was run in a 6.5 l airlift bioreactor with a concentric draft tube using lactose as substrate. Data on the operation with semi-synthetic medium with 50 and 100 g/l initial lactose concentrations are presented. The best result for -galactosidase productivity… CONTINUE READING