Aspergillus niger Prolyl Endoprotease for Hydrogen–Deuterium Exchange Mass Spectrometry and Protein Structural Studies

@inproceedings{Tsiatsiani2017AspergillusNP,
  title={Aspergillus
niger Prolyl Endoprotease
for Hydrogen–Deuterium Exchange Mass Spectrometry and Protein
Structural Studies},
  author={Liana Tsiatsiani and Michiel Akeroyd and Maurien M A Olsthoorn and Albert J. R. Heck},
  booktitle={Analytical chemistry},
  year={2017}
}
To monitor the structural integrity of therapeutic proteins, hydrogen-deuterium exchange mass spectrometry (HDX-MS) is increasingly utilized in the pharmaceutical industry. The successful outcome of HDX-MS analyses depends on the sample preparation conditions, which involve the rapid digestion of proteins at 0 °C and pH 2.5. Very few proteases are able to withstand such harsh conditions, with pepsin being the best-known exception, even though its activity is also strongly reduced at 0 °C. Here… CONTINUE READING
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