Aspergillus niger PA2 Tyrosinase Covalently Immobilized on a Novel Eco-Friendly Bio-Composite of Chitosan-Gelatin and Its Evaluation for L-DOPA Production

@article{Agarwal2016AspergillusNP,
  title={Aspergillus niger PA2 Tyrosinase Covalently Immobilized on a Novel Eco-Friendly Bio-Composite of Chitosan-Gelatin and Its Evaluation for L-DOPA Production},
  author={Pragati Agarwal and Swati Dubey and Mukta Singh and Rajesh P. Singh},
  journal={Frontiers in Microbiology},
  year={2016},
  volume={7}
}
Tyrosinase (EC 1.14.18.1) a copper-containing monooxygenase, isolated from a fungal isolate Aspergillus niger PA2 was subjected for immobilization onto a composite consisting of chitosan and gelatin biopolymers. The homogeneity of the chitosan-gelatin biocomposite film was characterized by X-ray diffraction analyses. To evaluate immobilization efficiency, chitosan-gelatin-Tyr bio-composite films were analyzed by field emission scanning electron microscopy, atomic force microscopy and UV… 
View on PubMed
frontiersin.org
7 Citations
Background Citations
3

Figures and Tables from this paper

7 Citations

Citation Type

Citation Type

Metal ion effects on Polyphenol Oxidase Covalently immobilized on a Bio-Composite.
TLDR
The properties of immobilized enzyme were investigated and compared to free enzyme, various parameters were studied such as pH, temperature and storage stability on immobilized and free enzyme and inhibition types of metal ions were determined.
Optimization and Characterization of Tyrosinases from Multi-enzyme Producing Fusarium solani and Fumago sp.
Tyrosinase is a copper-containing metalloprotein that catalyzes the oxidation of tyrosine, in particular, L-DOPA to L-Dopaquinone, which are precursors of brown pigments in some wounded eukaryotic…
A perspective on the biotechnological applications of the versatile tyrosinase.
Microbial Tyrosinases: A Novel Enzyme, Structural Features, and Applications
Phenolic-compounds sensor based on immobilization of tyrosinase in polyacrylamide gel on long-period fiber grating
Tyrosinase mediated humic substances synthesis by Bacillus aryabhattai TFG5
TLDR
Spectroscopic evidences suggest that TyrB enhanced the HS production from coir pith biomass and degradative products and changes in biomass structure by TyrB analysed through FT-IR suggests thatyrB might follow the polyphenol theory of HS synthesis.
Effect of heat-treated Nuruk on the quality characteristics of aged Yakju
TLDR
Sensory evaluation indicated that bad taste attributes were higher in Yakju produced using non-heat-treated Nuruk, and heat treatment of Nuruk at 50 °C can be adopted as a method for improving Yakju quality, as enzymatic activities that affect color, aroma, and taste are regulated.

References

SHOWING 1-10 OF 56 REFERENCES
Immobilization of tyrosinase on modified diatom biosilica: enzymatic removal of phenolic compounds from aqueous solution.
Stability and properties of mushroom tyrosinase entrapped in alginate, polyacrylamide and gelatin gels
Efficient Agaricus bisporus tyrosinase immobilization on cellulose-based carriers
A mediator-free phenol biosensor based on immobilizing tyrosinase to ZnO nanoparticles.
Immobilization of laccase on itaconic acid grafted and Cu(II) ion chelated chitosan membrane for bioremediation of hazardous materials
TLDR
Flexibility of the enzyme immobilized grafted polymer chains is expected to provide easy reaction conditions without diffusion limitation for substrate dye molecules and their products.
Development of electrochemical biosensor based on tyrosinase immobilized in composite biopolymeric film.
Amperometric biosensor based on tyrosinase-conjugated polysaccharide hybrid film: selective determination of nanomolar neurotransmitters metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC) in biological fluid.
Biofuel cell and phenolic biosensor based on acid-resistant laccase-glutaraldehyde functionalized chitosan-multiwalled carbon nanotubes nanocomposite film.
Microplate based optical biosensor for L-Dopa using tyrosinase from Amorphophallus campanulatus.
Synthesis and characterization of a novel chitosan-gelatin bioconjugate with fluorescence emission.
  • F. Mi
  • Chemistry, Biology
    Biomacromolecules
  • 2005
TLDR
It is revealed that chitosan could react with genipin via a nucleophilic ring-opening reaction to construct more sufficient and extensive cross-link networks, as compared with its gelatin counterpart, which may provide a novel way to deliver therapeutic radionuclides for immuno-targeting purposes in the future.
...
1
2
3
4
5
...