Aspergillus as a host for heterologous protein production: the problem of proteases.

@article{Hombergh1997AspergillusAA,
  title={Aspergillus as a host for heterologous protein production: the problem of proteases.},
  author={J P van den Hombergh and Peter J. I. van de Vondervoort and Laurence Fraissinet-Tachet and J. Arjan G. M. de Visser},
  journal={Trends in biotechnology},
  year={1997},
  volume={15 7},
  pages={256-63}
}
Homologous and heterologous protein production by filamentous fungi is often limited by the expression of proteases at high levels. By eliminating specific protease activities, protein production in Aspergillus niger can be improved considerably. Both classical mutagenesis and gene disruption techniques have yielded strains with reduced protease expression. Combinations of these mutations and disruptions result in a further reduction of protease activity. The coupling of efficient promoters to… CONTINUE READING

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