Aspartic-129 is an essential residue in the catalytic mechanism of the low M(r) phosphotyrosine protein phosphatase.

@article{Taddei1994Aspartic129IA,
  title={Aspartic-129 is an essential residue in the catalytic mechanism of the low M(r) phosphotyrosine protein phosphatase.},
  author={Niccol{\`o} Taddei and Paola Chiarugi and Paolo Cirri and Tania Fiaschi and Massimo Stefani and Guido Camici and Giovanni Raugei and G. Manao G. Ramponi},
  journal={FEBS letters},
  year={1994},
  volume={350 2-3},
  pages={328-32}
}
The crystal structure of the bovine liver low M(r) phosphotyrosine protein phosphatase suggests the involvement of aspartic acid-129 in enzyme catalysis. The Asp-129 to alanine mutant has been prepared by oligonucleotide-directed mutagenesis of a synthetic gene coding for the enzyme. The purified mutant elicited an highly reduced specific activity (about 0.04% of the activity of the wild-type) and a native-like fold, as judged by 1H NMR spectroscopy. The kinetic analysis revealed that the… CONTINUE READING