Asparaginase Ii-gfp Fusion as a Tool for Studying the Secretion of the Enzyme under Nitrogen Starvation

Abstract

Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II… (More)

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Cite this paper

@inproceedings{SoteroMartins2004AsparaginaseIF, title={Asparaginase Ii-gfp Fusion as a Tool for Studying the Secretion of the Enzyme under Nitrogen Starvation}, author={Adriana Sotero-Martins and Elba Pinto da Silva Bon and Elvira Carvajal and Maria Deane}, year={2004} }