Asp578 in LEU4p is one of the key residues for leucine feedback inhibition release in sake yeast.

@article{Oba2005Asp578IL,
  title={Asp578 in LEU4p is one of the key residues for leucine feedback inhibition release in sake yeast.},
  author={Takahiro Oba and Shuji Nomiyama and Hideki Hirakawa and Kousuke Tashiro and Satoru Kuhara},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2005},
  volume={69 7},
  pages={1270-3}
}
We identified a new mutation, Asp578Tyr, in alpha-isopropylmalate synthase (a LEU4 gene product) that releases leucine feedback inhibition and causes hyperproduction of isoamyl alcohol (i-AmOH) in sake yeast. Spontaneous sake yeast mutants that express resistance to 5,5,5-trifluoro-DL-leucine (TFL) were isolated, and a mutant strain, TFL20, was characterized at the genetic and biochemical levels. An enzyme assay for alpha-isopropylmalate synthase showed that strain TFL20 was released from… CONTINUE READING

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