Asp-70-->Lys mutant of factor X lacks high affinity Ca2+ binding site yet retains function.

Abstract

To study the role of a putative high affinity Ca2+ binding site in the protease domain of factor X, we prepared a deletion mutant (E2FX) lacking the Gla and first epidermal growth factor-like domains. E2FX possesses a single high affinity Ca2+ binding site (Kd = 154 microM). Asp-70 or Glu-80 (chymotrypsin numbering system) was then converted to Lys. These… (More)

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Cite this paper

@article{Rezaie1994Asp70LysMO, title={Asp-70-->Lys mutant of factor X lacks high affinity Ca2+ binding site yet retains function.}, author={Anahita Rezaie and Charles T. Esmon}, journal={The Journal of biological chemistry}, year={1994}, volume={269 34}, pages={21495-9} }