Ash1p is a site-specific DNA-binding protein that actively represses transcription.

  title={Ash1p is a site-specific DNA-binding protein that actively represses transcription.},
  author={Mary E. Maxon and Ira Herskowitz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={98 4},
ASH1 encodes a protein that is localized specifically to the daughter cell nucleus, where it has been proposed to repress transcription of the HO gene. Using Ash1p purified from baculovirus-infected insect cells, we have shown that Ash1p binds specific DNA sequences in the HO promoter. DNase I protection analyses showed that Ash1p recognizes a consensus sequence, YTGAT. Mutation of this consensus abolishes Ash1p DNA binding in vitro. We have shown that Ash1p requires an intact zinc-binding… CONTINUE READING


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