Ash1p is a site-specific DNA-binding protein that actively represses transcription.

@article{Maxon2001Ash1pIA,
  title={Ash1p is a site-specific DNA-binding protein that actively represses transcription.},
  author={Mary E. Maxon and Ira Herskowitz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2001},
  volume={98 4},
  pages={1495-500}
}
ASH1 encodes a protein that is localized specifically to the daughter cell nucleus, where it has been proposed to repress transcription of the HO gene. Using Ash1p purified from baculovirus-infected insect cells, we have shown that Ash1p binds specific DNA sequences in the HO promoter. DNase I protection analyses showed that Ash1p recognizes a consensus sequence, YTGAT. Mutation of this consensus abolishes Ash1p DNA binding in vitro. We have shown that Ash1p requires an intact zinc-binding… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-4 of 4 references

The Molecular and Cellular Biology of the Yeast Saccharomyces cerevisiae: Gene Expression

I. Herskowitz, J. Rine, J. Strathern
1992

Methods in Yeast Genetics: A Laboratory Course Manual (Cold

M. D. Rose, F. Winston, P. Hieter
1990

Molecular Cloning: A Laboratory Manual (Cold

J. Sambrook, E. F. Fritsch, T. Maniatis
1989

Antibodies: A Laboratory Manual (Cold

E. Harlow, D. Lane
1988

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