Ascorbate protects endothelial barrier function during septic insult: Role of protein phosphatase type 2A.

@article{Han2010AscorbatePE,
  title={Ascorbate protects endothelial barrier function during septic insult: Role of protein phosphatase type 2A.},
  author={Min Ho Han and Suresh Pendem and Suet Ling Teh and Dinesh K. Sukumaran and Feng Wu and John X. Wilson},
  journal={Free radical biology & medicine},
  year={2010},
  volume={48 1},
  pages={128-35}
}
Endothelial barrier dysfunction contributes to morbidity in sepsis. We tested the hypothesis that raising the intracellular ascorbate concentration protects the endothelial barrier from septic insult by inhibiting protein phosphatase type 2A. Monolayer cultures of microvascular endothelial cells were incubated with ascorbate, dehydroascorbic acid (DHAA), the NADPH oxidase inhibitors apocynin and diphenyliodonium, or the PP2A inhibitor okadaic acid and then were exposed to septic insult… CONTINUE READING