Ascorbate free-radical reduction by glyoxysomal membranes.

@article{Bowditch1990AscorbateFR,
  title={Ascorbate free-radical reduction by glyoxysomal membranes.},
  author={M L Bowditch and Robert Paul Donaldson},
  journal={Plant physiology},
  year={1990},
  volume={94 2},
  pages={
          531-7
        }
}
Glyoxysomal membranes from germinating castor bean (Ricinus communis L. cv Hale) endosperm contain an NADH dehydrogenase. This enzyme can utilize extraorganellar ascorbate free-radical as a substrate and can oxidize NADH at a rate which can support intraglyoxysomal demand for NAD(+). NADH:ascorbate free-radical reductase was found to be membrane-associated, and the activity remained in the membrane fraction after lysis of glyoxysomes by osmotic shock, followed by pelleting of the membranes. In… CONTINUE READING

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