Ascorbate and alpha-tocopherol prevent apoptosis induced by serum removal independent of Bcl-2.

@article{Barroso1997AscorbateAA,
  title={Ascorbate and alpha-tocopherol prevent apoptosis induced by serum removal independent of Bcl-2.},
  author={Michel Peter Barchik Salvatierra Barroso and Consuelo G{\'o}mez-D{\'i}az and Guillermo L{\'o}pez-Lluch and Mar{\'i}a Del Mar Malag{\'o}n and Frederick L. Crane and Pl{\'a}cido Navas},
  journal={Archives of biochemistry and biophysics},
  year={1997},
  volume={343 2},
  pages={243-8}
}
Cells require serum to maintain growth in vitro. Serum provides growth and survival factors and its removal causes an oxidative stress that induces peroxidations in membrane lipids and development of programmed cell death (apoptosis) in some cells. Cells containing Bcl-2 are partially protected against both lipid peroxidation and apoptosis and some cell lines, such as Daudi, which lack this protein, are very sensitive to serum removal. Thus, cells are grown for 48 h in the absence of fetal calf… CONTINUE READING