Ascorbate-Fe2+ lipid-peroxidation of rat liver microsomes: Effect of vitamin E and cytosolic proteins

  title={Ascorbate-Fe2+ lipid-peroxidation of rat liver microsomes: Effect of vitamin E and cytosolic proteins},
  author={R. Zanetti and {\'A}. Catala},
  journal={Molecular and Cellular Biochemistry},
In the present study, we examined the effect of the intraperitoneal administration of vitamin E (100 mg/kg weight/24 h) on ascorbate (0.4 mM) induced lipid peroxidation of rat liver microsomes . We also analyzed the effect of hepatic cytosolic proteins on this process. The results indicate that the ascorbate induced light emission was 76% lower in microsomes (1 mg protein) obtained from vitamin E treated animals when compared with controls. In the presence of cytosolic protein (1 mg) the… Expand
The protection of long chain polyunsaturated fatty acids by melatonin during nonenzymatic lipid peroxidation of rat liver microsomes
It is indicated that melatonin may act in vivo and in vitro as an antioxidant protecting long chain PUFA present in rat liver microsomes from the deleterious effect by a selective mechanism that reduces the loss of docosahexaenoic and arachidonic acids. Expand
The effect of α-tocopherol on the lipid peroxidation of mitochondria and microsomes obtained from rat liver and testis
The treatment with α‐tocopherol at the dose and times indicated showed a protector effect on the polyunsaturated fatty acids of liver mitochondria, microsomes and testis mitochondrial, whereas those fatty acids situated in testis microsome were not protected during non enzymatic ascorbate‐Fe2+ lipid peroxidation. Expand
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Melatonin preserves arachidonic and docosapentaenoic acids during ascorbate-Fe2+ peroxidation of rat testis microsomes and mitochondria.
The protective effect observed by melatonin in rat testis mitochondria was higher than that observed in microsomes which could be explained if the sum of C20:4 n6+C22:5 n6 in testis microsome is two-fold greater than present in mitochondria. Expand
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The effect of α tocopherol, all-trans retinol and retinyl palmitate on the non enzymatic lipid peroxidation induced by ascorbate-Fe2+ of rod outer segment membranes isolated from bovine retina wasExpand
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  • Á. Catala
  • Chemistry, Medicine
  • The international journal of biochemistry & cell biology
  • 2006
The author proposes the outer segments of photoreceptors as an excellent model to study lipid peroxidation using the chemiluminescence assay since these membranes contain the highest concentration of polyunsaturated fatty acids of any vertebrate tissue and are highly susceptible to oxidative damage. Expand
Five Decades with Polyunsaturated Fatty Acids: Chemical Synthesis, Enzymatic Formation, Lipid Peroxidation and Its Biological Effects
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Ascorbate-dependent capacity of dialysed rat liver cytosol to prevent nonenzymatic lipid peroxidation.
The capacity of rat liver cytosol to decrease Fe/ADP ascorbate-induced lipid peroxidation in microsomes was evaluated using the chemiluminescence technique and measuring the formation ofExpand
Superoxide-dependent lipid peroxidation and vitamin E content of microsomes from hepatomas with different growth rates.
It is proposed that the high resistance to superoxide-dependent lipid peroxidation of hepatoma 3924A microsomes is related to the low substrate availability rather than the content of membrane antioxidants, and would be due to the partial deficiency of the endogenous propagating agent, cytochrome P-450. Expand
The relationship between chemiluminescence and lipid peroxidation in rat hepatic microsomes.
The results of these experiments indicate that chemiluminescence initiated in hepatic microsomes by either NADPH or ascorbate is directly proportional to lipid peroxidation. Expand
Some basic properties of ascorbate-dependent antioxidative-defence factors from rat liver cytosol.
Differences were observed in pH-dependence, though both factors were more active in neutral media (pH 6.2-6.8), and a function of these factors could be to create more favourable conditions for the interaction of ascorbate as a very hydrophilic molecule with membrane-bound hydrophobic radicals. Expand
Vitamin E: interactions with free radicals and ascorbate.
  • P. McCay
  • Chemistry, Medicine
  • Annual review of nutrition
  • 1985
It seems clear that the biological function of this vitamin is not specific in the sense of its being a cofactor for an enzymic reaction (as is the case for the B-complex vitamins), and essentially any tissue can be made to develop characteristic signs as a result of a deficiency of this vitamins. Expand
Dietary vitamin E decreases ESR signal intensity in hepatic microsomal preparations from malignant hyperthermia susceptible pigs.
Vitamin E supplementation may decrease the peroxidative events associated with MH and Elevated pyruvate kinase activities and TBARS concentrations in plasma of MHS pigs were moderated by dietary vitamin E. Expand
Antioxidative effect of α-tocopherol incorporation into lecithin liposomes on ascorbic acid-Fe2+-induced lipid peroxidation
Abstract The antioxidative effect of α-tocopherol incorporated into lecithin liposomes was studied. Lipid peroxidation of liposome membranes, assayed as malondialdehyde production, was catalyzed byExpand
Is vitamin E the only lipid-soluble, chain-breaking antioxidant in human blood plasma and erythrocyte membranes?
It is concluded that vitamin E, which is largely present as alpha-tocopherol, is the only significant lipid-soluble, chain-breaking type of antioxidant present in human blood. Expand
Identification of a low molecular mass (14.2 kDa) alpha-tocopherol-binding protein in the cytosol of rat liver and heart.
The TBP stimulated the transfer of d alpha-tocopherol from liposomes to mitochondria in vitro by 8-10 fold, suggesting that low molecular mass TBPs may play a role in intracellular vitamin E transport. Expand
Membrane characteristics in vitamin E deficiency and the assessment of vitamin E status.
The chemical anatomy of the membrane is described in ‘membrane models’, the most recent being that of Singer & Nicolson (1972). Expand