Artificial insertion of peptides between signal peptide and mature protein: effect on secretion and processing of hybrid thermostable alpha-amylases in Bacillus subtilis and Escherichia coli cells.

@article{Itoh1990ArtificialIO,
  title={Artificial insertion of peptides between signal peptide and mature protein: effect on secretion and processing of hybrid thermostable alpha-amylases in Bacillus subtilis and Escherichia coli cells.},
  author={Yohjiro Itoh and Kaneo Kanoh and Kazuo Nakamura and Kazuma Takase and Kunio Yamane},
  journal={Journal of general microbiology},
  year={1990},
  volume={136 8},
  pages={1551-8}
}
To study the effect of inserted peptides on the secretion and processing of exported proteins in Bacillus subtilis and Escherichia coli, pBR322-derived DNA fragments coding for small peptides were inserted between the DNA coding for the 31 amino acid B. subtilis alpha-amylase signal peptide and that coding for the mature part of the extracellular… CONTINUE READING