Artificial fertilization by intracytoplasmic sperm injection in a teleost fish, the medaka (Oryzias latipes).


Intracytoplasmic sperm injection (ICSI) is a technique that has been successfully used for assisting reproduction in mammals. However, this method is still not reliable in nonmammalian species, including teleosts. We succeeded in producing medaka individuals by ICSI with a rate of 13.4% (28 hatched embryos out of 209 eggs fertilized by ICSI), the best value… (More)
DOI: 10.1095/biolreprod.108.069880