Artificial control of transgene expression in Chlamydomonas reinhardtii chloroplast using the lac regulation system from Escherichia coli.

@article{Kato2007ArtificialCO,
  title={Artificial control of transgene expression in Chlamydomonas reinhardtii chloroplast using the lac regulation system from Escherichia coli.},
  author={Ko Kato and Takahiro Marui and Seitaro Kasai and Astuhiko Shinmyo},
  journal={Journal of bioscience and bioengineering},
  year={2007},
  volume={104 3},
  pages={207-13}
}
Systems that can control the expression of a gene both temporally and spatially are important for the study of transgenic plants. Here, we describe an artificial, controllable gene expression system using the lac regulation system from Escherichia coli that we constructed in the Chlamydomonas reinhardtii chloroplast. This system consists of a controllable reporter gene expression cassette and the Lac repressor expression cassette. We created controller promoters by modifying two promoter… CONTINUE READING