Artifactual-free analysis of S-nitrosoglutathione and S-nitroglutathione by neutral-pH, anion-pairing, high-performance liquid chromatography. Study on peroxynitrite-mediated S-nitration of glutathione to S-nitroglutathione under physiological conditions.

  title={Artifactual-free analysis of S-nitrosoglutathione and S-nitroglutathione by neutral-pH, anion-pairing, high-performance liquid chromatography. Study on peroxynitrite-mediated S-nitration of glutathione to S-nitroglutathione under physiological conditions.},
  author={Dimitrios Tsikas and Katrin Denker and J{\"u}rgen C. Fr{\"o}lich},
  journal={Journal of chromatography. A},
  volume={915 1-2},

Carbonic anhydrases are producers of S-nitrosothiols from inorganic nitrite and modulators of soluble guanylyl cyclase in human platelets

This study provides the first evidence that extra-platelet nitrite and erythrocytic CAII may modulate platelet function in a cGMP-dependent manner and proposes that nitrous acid (ONOH) is the primary CA-catalyzed reaction product of nitrite.

Oxidation and Nitrosation of Thiols at Low Micromolar Exposure to Nitric Oxide

The data indicate that RSNO formation is favored at oxygen concentrations that typically occur in tissues, and that nitrosothiol formation in vivo depends not only on the availability of ⋅NO and O2 but also on the degree of oxidative stress by affecting the steady-state concentration of thiyl radicals.

Redox-Sensitivity and Site-Specificity of S- and N- Denitrosation in Proteins

Overall, the accessibility of Trp residues to redox-active biomolecules determines the stability of protein-associated nitroso species such that in the case of HSA, N-nitroso-Trp-214 is insensitive to denitrosation by low-molecular-weight antioxidants.

GC-MS and HPLC methods for peroxynitrite (ONOO- and O15NOO-) analysis: a study on stability, decomposition to nitrite and nitrate, laboratory synthesis, and formation of peroxynitrite from S-nitrosoglutathione (GSNO) and KO2.

The GC-MS and HPLC methods were used to study stability, synthesis, formation from S-[(15)N]nitrosoglutathione and KO(2), and isomerization/decomposition of peroxynitrite to NO(2)(-) and NO(3)(-) in aqueous buffer.



S-Nitroglutathione, a Product of the Reaction between Peroxynitrite and Glutathione That Generates Nitric Oxide*

Reactions of ONOO− with GSH resulted in the generation of NO, which was detected by the head space/NO-chemiluminescence analyzer method and was inhibited by the presence of glucose and/or CO2 in the buffers employed.

Investigations of S-transnitrosylation reactions between low- and high-molecular-weight S-nitroso compounds and their thiols by high-performance liquid chromatography and gas chromatography-mass spectrometry.

The results suggest that the mechanism of S-transnitrosylation reactions of these S-nitroso compounds and their thiols involve heterolytic cleavage of the S&sbond;N bond.

S‐Nitrosoglutathione in Rat Cerebellum: Identification and Quantification by Liquid Chromatography‐Mass Spectrometry

The HPLC analysis of cerebellar extract from [35S]cysteine‐prelabeled slices suggested that S‐nitrosoglutathione (GSNO) was indeed present in rat brain and was the first direct demonstration of the presence of endogenous GSNO in CNS.

High-performance liquid chromatography of glutathione conjugates

High-performance liquid chromatographic method based on reversed-phase ion-pair chromatography applied to the determination of GSH S-transferase activity in the liver cytosol of rat, rabbit and man towards different electrophilic acceptors correlated well with results obtained by previously reported spectrophotometric assay methods.

A New Pathway of Nitric Oxide/Cyclic GMP Signaling InvolvingS-Nitrosoglutathione*

Cu+-specific chelators inhibited bradykinin-induced cGMP release from rat isolated hearts but did not interfere with the direct activation of cardiac sGC, suggesting that thionitrites may occur as intermediates of NO/cGMP signaling in mammalian tissues.

Simultaneous derivatization and quantification of the nitric oxide metabolites nitrite and nitrate in biological fluids by gas chromatography/mass spectrometry.

  • D. Tsikas
  • Chemistry, Biology
    Analytical chemistry
  • 2000
A GC/MS method was developed which allows, for the first time, simultaneous quantification of nitrite and nitrate in various biological fluids and enables discrimination between S-nitro- and S-Nitroso-glutathione, which have identical chromatographic and spectrophotometric properties.

Acceleration of peroxynitrite oxidations by carbon dioxide.

It is proposed that O=N-OOCO 2- rearranges to give a nitrocarbonate anion, O2N-OCO2- which in turn, may serve as the proximal oxidant in biological systems that produce peroxynitrite.

The oxidative and nitrosative chemistry of the nitric oxide/superoxide reaction in the presence of bicarbonate.

It was concluded that NO reacts with both trans-HOONO and a CO2 adduct of ONOO- to form nitrosating species which have similar oxidation chemistry and reactivity with and NO.

Measurement of nitrite and nitrate in biological fluids by gas chromatography-mass spectrometry and by the Griess assay: problems with the Griess assay--solutions by gas chromatography-mass spectrometry.

Further interferences by free reduced thiols, proteins, and other plasma constituents in the Griess assay but not in GC-MS are reported, which are associated with many problems in measuring nitrate in biological fluids.

Peroxynitrite: an endogenous oxidizing and nitrating agent

This complex chemistry can be explained by the formation of several structural forms and active intermediates released from peroxynitrite.