Artifactual-free analysis of S-nitrosoglutathione and S-nitroglutathione by neutral-pH, anion-pairing, high-performance liquid chromatography. Study on peroxynitrite-mediated S-nitration of glutathione to S-nitroglutathione under physiological conditions.
@article{Tsikas2001ArtifactualfreeAO, title={Artifactual-free analysis of S-nitrosoglutathione and S-nitroglutathione by neutral-pH, anion-pairing, high-performance liquid chromatography. Study on peroxynitrite-mediated S-nitration of glutathione to S-nitroglutathione under physiological conditions.}, author={Dimitrios Tsikas and Katrin Denker and J{\"u}rgen C. Fr{\"o}lich}, journal={Journal of chromatography. A}, year={2001}, volume={915 1-2}, pages={ 107-16 } }
36 Citations
UPLC-MS/MS measurement of S-nitrosoglutathione (GSNO) in human plasma solves the S-nitrosothiol concentration enigma.
- BiologyJournal of chromatography. B, Analytical technologies in the biomedical and life sciences
- 2013
Oxidation and Nitrosation of Thiols at Low Micromolar Exposure to Nitric Oxide
- Chemistry, BiologyThe Journal of Biological Chemistry
- 2003
The data indicate that RSNO formation is favored at oxygen concentrations that typically occur in tissues, and that nitrosothiol formation in vivo depends not only on the availability of ⋅NO and O2 but also on the degree of oxidative stress by affecting the steady-state concentration of thiyl radicals.
Determination of GSH, GSSG, and GSNO using HPLC with electrochemical detection.
- BiologyMethods in enzymology
- 2010
Redox-Sensitivity and Site-Specificity of S- and N- Denitrosation in Proteins
- Biology, ChemistryPloS one
- 2010
Overall, the accessibility of Trp residues to redox-active biomolecules determines the stability of protein-associated nitroso species such that in the case of HSA, N-nitroso-Trp-214 is insensitive to denitrosation by low-molecular-weight antioxidants.
S-Transnitrosation reactions of hydrogen sulfide (H2S/HS-/S2-) with S-nitrosated cysteinyl thiols in phosphate buffer of pH 7.4: Results and review of the literature.
- ChemistryNitric oxide : biology and chemistry
- 2017
Ultra-trace determination of S-nitrosothiols in blood samples by spectrofluorimetry with 8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene.
- ChemistrySpectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- 2008
Sensitive determination of S-nitrosothiols in human blood by spectrofluorimetry using a fluorescent probe: 1,3,5,7-tetramethyl-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene.
- ChemistryTalanta
- 2007
GC-MS and HPLC methods for peroxynitrite (ONOO- and O15NOO-) analysis: a study on stability, decomposition to nitrite and nitrate, laboratory synthesis, and formation of peroxynitrite from S-nitrosoglutathione (GSNO) and KO2.
- ChemistryThe Analyst
- 2011
The GC-MS and HPLC methods were used to study stability, synthesis, formation from S-[(15)N]nitrosoglutathione and KO(2), and isomerization/decomposition of peroxynitrite to NO(2)(-) and NO(3)(-) in aqueous buffer.
Oxidation and nitrosylation of oxyhemoglobin by S-nitrosoglutathione via nitroxyl anion.
- Biology, ChemistryFree radical biology & medicine
- 2003
References
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Reactions of ONOO− with GSH resulted in the generation of NO, which was detected by the head space/NO-chemiluminescence analyzer method and was inhibited by the presence of glucose and/or CO2 in the buffers employed.
S‐Nitrosoglutathione in Rat Cerebellum: Identification and Quantification by Liquid Chromatography‐Mass Spectrometry
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The HPLC analysis of cerebellar extract from [35S]cysteine‐prelabeled slices suggested that S‐nitrosoglutathione (GSNO) was indeed present in rat brain and was the first direct demonstration of the presence of endogenous GSNO in CNS.
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High-performance liquid chromatographic method based on reversed-phase ion-pair chromatography applied to the determination of GSH S-transferase activity in the liver cytosol of rat, rabbit and man towards different electrophilic acceptors correlated well with results obtained by previously reported spectrophotometric assay methods.
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Cu+-specific chelators inhibited bradykinin-induced cGMP release from rat isolated hearts but did not interfere with the direct activation of cardiac sGC, suggesting that thionitrites may occur as intermediates of NO/cGMP signaling in mammalian tissues.
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A GC/MS method was developed which allows, for the first time, simultaneous quantification of nitrite and nitrate in various biological fluids and enables discrimination between S-nitro- and S-Nitroso-glutathione, which have identical chromatographic and spectrophotometric properties.
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It is proposed that O=N-OOCO 2- rearranges to give a nitrocarbonate anion, O2N-OCO2- which in turn, may serve as the proximal oxidant in biological systems that produce peroxynitrite.
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It was concluded that NO reacts with both trans-HOONO and a CO2 adduct of ONOO- to form nitrosating species which have similar oxidation chemistry and reactivity with and NO.
Peroxynitrite: an endogenous oxidizing and nitrating agent
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This complex chemistry can be explained by the formation of several structural forms and active intermediates released from peroxynitrite.
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