Artifacts associated with mithramycin fluorescence in the clinical detection and quantitation of aneuploidy by flow cytometry.

@article{Cunningham1982ArtifactsAW,
  title={Artifacts associated with mithramycin fluorescence in the clinical detection and quantitation of aneuploidy by flow cytometry.},
  author={Robert E. Cunningham and K S Skramstad and A E Newburger and Stanley Shackney},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1982},
  volume={30 4},
  pages={317-22}
}
Ethanol-fixed cells stored at 4 degrees C exhibit fixation time-dependent hyperchromatism in comparison with freshly fixed cells when stained with mithramycin and examined by flow cytometry. This hyperchromatism has been found to be temperature-dependent, developing fully within 72 hr at room temperature, and within 2 hr at 37 degrees C. Cells from normal donors that are stained with mithramycin exhibit spurious aneuploid peaks. These spurious aneuploid peaks can be eliminated by incubating… CONTINUE READING

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