Articular cartilage pharmacology: I. In vitro studies on glucosamine and non steroidal antiinflammatory drugs.

  title={Articular cartilage pharmacology: I. In vitro studies on glucosamine and non steroidal antiinflammatory drugs.},
  author={R R Vidal y Plana and D. Bizzarri and A. L. Rovati},
  journal={Pharmacological research communications},
  volume={10 6},

Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro.

GS modified cultured OA chondrocyte metabolism by acting on PKC, cellular PLA2, protein synthesis and possibly collagenase activation, and significantly and dose-dependently increased protein synthesis.

Glucosamine and chondroitin sulfates in the treatment of osteoarthritis: a survey.

Experimental evidence indicates that glucosamine sulfate and chondroitin sulfate have a particular tropism for cartilage where they serve as substrates in the biosynthesis of component building blocks.

High doses of glucosamine-HCl have detrimental effects on bovine articular cartilage explants cultured in vitro.

It is shown that pharmacological doses of glucosamine induce a broad impairment in the metabolic activity of bovine chondrocytes, leading to cell death, and particular attention should be addressed to the experimental model, the doses and the length of treatment.

Sulfated glycosaminoglycans and glucosamine may synergize in promoting synovial hyaluronic acid synthesis.

The possibility that oral glucosamine and CS may interact in a complementary or synergistic fashion to improve synovial fluid HA content in OA should be assessed in clinical studies, and the potential of adjunctive CS administration to improve the clinical response achievable with optimal intakes of glucose should likewise be evaluated.

Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro.

Glucosamine sulfate did not affect DNA synthesis nor coll II production but caused a statistically significant stimulation of PG production by chondrocytes from human osteoarthritic cartilage cultured for up to 12 days in 3-dimensional cultures.

Comparison of glucose derivatives effects on cartilage degradation

It is shown that glucosamine derivatives can alter anabolic and catabolic processes in HACs induced by IL-1β, and GlcN-S had the highest chondroprotective activity of all four chemicals.

Niacinamide therapy for osteoarthritis--does it inhibit nitric oxide synthase induction by interleukin 1 in chondrocytes?

Non-toxic nutritional regimens, by intervening at multiple points in the signal transduction pathways that promote the synthesis and mediate the activity of IL-1, may provide a substantially superior alternative to NSAIDs in the treatment and perhaps prevention of OA.



Effect of anti-inflammatory drugs on glucosamine-6-phosphate synthetase from inflamed tissue of rats.

Good correlation was seen between increases of the enzyme activity and the swelling in the hind paws as adjuvant disease appeared and developed in rats and the drug-action to Gm-6-P synthesis with the paw enzyme was much clearer in the reaction at pH 6.8 than pH 7.7.

The importance of amino acids as dialyzable components of rat serum which promote sulfate uptake by cartilage from hypophysectomized rats in vitro.

It is suggested that a part of the dialyzable sulfation-promoting activity of serum is attributable to amino acids, and an interaction between the nondialyzable, probably protein or protein-bound, component and amino acids is also suggested.

Acid mucopolysaccharides in calcified tissues.

Studies on mechanism of action of salicylates. V. Effect of salicylic acid on enzymes involved in mucopolysaccharides synthesis.

The wound-healing retardation action of salicylates is probably due mainly to its inhibitory action on mucopolysaccharide synthesis.

Glutamine as an Accelerator of Chondroitin Sulphate Synthesis

In the course of the work, it was noted that small amounts of a liver homogenate added to the suspending medium produced a remarkable stimulation of the sulphate incorporation into chondroitin sulphuric acid.

The biosynthesis of intestinal mucins. The effect of salicylate on glycoprotein biosynthesis by sheep colonic and human gastric mucosal tissues in vitro.

An inhibitory effect of salicylate on glycoprotein biosynthesis at the level of the amino sugar intermediates is suggested by incubation of sheep colonic mucosal scrapings in Krebs-Ringer buffer and analysis of papain-digested glycop Protein.

Studies on the mechanism of action of salicylates. VII. Effect of a few anti-inflammatory agents on uridine-5'-diphosphoglucose dehydrogenase.

Phenylbutazone, oxyphenbutazone, indomeihacin, flufenamic acid, and mefenamic acid, like salicylic acid, inhibit the oxidation of uridine-5′-diphosphoglucose (UDPG) competitively with nicotinamide

A modified uronic acid carbazole reaction.