Arrest of replication by mammalian DNA polymerases alpha and beta caused by chromium-DNA lesions.

@article{Bridgewater1998ArrestOR,
  title={Arrest of replication by mammalian DNA polymerases alpha and beta caused by chromium-DNA lesions.},
  author={L C Bridgewater and Francis C.R. Manning and Steven R. Patierno},
  journal={Molecular carcinogenesis},
  year={1998},
  volume={23 4},
  pages={201-6}
}
We have previously shown that trivalent chromium, and hexavalent chromium in the presence of one of its primary in vivo reductants, ascorbate, can bind to DNA and form interstrand crosslinks capable of obstructing replication. This effect was demonstrated in vitro by using Sequenase Version 2.0 T7 DNA polymerase; its parent enzyme, the unmodified T7 DNA polymerase; and Escherichia coli polymerase I large (Klenow) fragment; and it was demonstrated ex vivo by using Taq polymerase and DNA from… CONTINUE READING

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