Array tomography: high-resolution three-dimensional immunofluorescence.

@article{Micheva2010ArrayTH,
  title={Array tomography: high-resolution three-dimensional immunofluorescence.},
  author={Kristina D. Micheva and Nancy A. O'Rourke and Brad Busse and Stephen J. Smith},
  journal={Cold Spring Harbor protocols},
  year={2010},
  volume={2010 11},
  pages={
          pdb.top89
        }
}
Array tomography, which is described in this article, is a volumetric microscopy method based on physical serial sectioning. Ultrathin sections of a plastic-embedded tissue are cut using an ultramicrotome, bonded in an ordered array to a glass coverslip, stained as desired, and imaged. The resulting two-dimensional image tiles can then be reconstructed computationally into three-dimensional volume images for visualization and quantitative analysis. The minimal thickness of individual sections… CONTINUE READING

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