Array-based high-throughput DNA markers for crop improvement

  title={Array-based high-throughput DNA markers for crop improvement},
  author={Piyush Kumar Gupta and Sachin Rustgi and R R Mir},
The last two decades have witnessed a remarkable activity in the development and use of molecular markers both in animal and plant systems. This activity started with low-throughput restriction fragment length polymorphisms and culminated in recent years with single nucleotide polymorphisms (SNPs), which are abundant and uniformly distributed. Although the latter became the markers of choice for many, their discovery needed previous sequence information. However, with the availability of… 

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This chapter describes the various DNA sequencing technologies, the strategies for discovery of SNP markers, and the low-to-medium-throughput approaches for SNP genotyping.

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Most SNPs used in this GoldenGate assay appear to be equally useful for diversity analysis, marker-trait association studies, and marker-aided breeding.

QTL scanning for rice yield using a whole genome SNP array.

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Diversity Arrays Technology can be effectively applied to genetic mapping and diversity analyses of barley and is highlighted as a generic technique for genome profiling in the context of molecular breeding and genomics.