Arrangement of glycan chains in the sacculus of Escherichia coli.

@article{Verwer1978ArrangementOG,
  title={Arrangement of glycan chains in the sacculus of Escherichia coli.},
  author={Ronald W. H. Verwer and Nanne Nanninga and W. Keck and Uli Schwarz},
  journal={Journal of bacteriology},
  year={1978},
  volume={136 2},
  pages={723-9}
}
A novel of Escherichia coli endopeptidase was used for a selective partial hydrolysis of the peptide bridges which interlink the glycan chains in E. coli sacculi. The loosening of the murein network revealed, in the electron microscope, a preferential orientation of the glycan chains, more or less perpendicular to the length axis of the cell. Control incubations with E. coli transglycosylase or egg-white lysozyme did not leave ordered structures behind.