Arrangement of cellular fibronectin in noncollagenous fibrils in human fibroblast cultures.

@article{Dzamba1991ArrangementOC,
  title={Arrangement of cellular fibronectin in noncollagenous fibrils in human fibroblast cultures.},
  author={Bette J Dzamba and Donna Peters},
  journal={Journal of cell science},
  year={1991},
  volume={100 ( Pt 3)},
  pages={605-12}
}
The assembly of fibronectin into fibrils was examined by high-voltage immunoelectron microscopy in subconfluent cultures of ascorbate-treated human skin fibroblasts. Cells grown in the presence of ascorbic acid for 24, 48 or 72 h were labeled with Ist-9, a monoclonal antibody specific for the EIIIA site in fibronectin, and polyclonal antibodies to type I collagen. Cells were then labeled with goat anti-mouse IgG and goat anti-rabbit IgG coupled to 5 or 18 nm colloidal gold beads. Our results… CONTINUE READING

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