Aromatase activity in the brain of the three-spined stickleback, Gasterosteus aculeatus. II. Effects of castration in winter.

@article{Borg1987AromataseAI,
  title={Aromatase activity in the brain of the three-spined stickleback, Gasterosteus aculeatus. II. Effects of castration in winter.},
  author={Bertil Borg and R. J. M. Timmers and Jan G.D. Lambert},
  journal={Experimental biology},
  year={1987},
  volume={47 2},
  pages={69-71}
}
Non-breeding stickleback males were gonadectomized or sham-operated in winter. Treatment with long photoperiod at high temperature stimulated sexual maturation in control fish. Brains were freeze-sectioned and punches were taken with hollow needles from different diencephalic regions and the punches incubated with [19-3H]-androstenedione. After extraction of steroids with CH2Cl2 tritium activity remaining in the water phase was measured. This gives a measure of aromatization as tritiated water… CONTINUE READING