Arl2- and Msps-dependent microtubule growth governs asymmetric division

Abstract

Asymmetric division of neural stem cells is a fundamental strategy to balance their self-renewal and differentiation. It is long thought that microtubules are not essential for cell polarity in asymmetrically dividing Drosophila melanogaster neuroblasts (NBs; neural stem cells). Here, we show that Drosophila ADP ribosylation factor like-2 (Arl2) and Msps, a known microtubule-binding protein, control cell polarity and spindle orientation of NBs. Upon arl2 RNA intereference, Arl2-GDP expression, or arl2 deletions, microtubule abnormalities and asymmetric division defects were observed. Conversely, overactivation of Arl2 leads to microtubule overgrowth and depletion of NBs. Arl2 regulates microtubule growth and asymmetric division through localizing Msps to the centrosomes in NBs. Moreover, Arl2 regulates dynein function and in turn centrosomal localization of D-TACC and Msps. Arl2 physically associates with tubulin cofactors C, D, and E. Arl2 functions together with tubulin-binding cofactor D to control microtubule growth, Msps localization, and NB self-renewal. Therefore, Arl2- and Msps-dependent microtubule growth is a new paradigm regulating asymmetric division of neural stem cells.

DOI: 10.1083/jcb.201503047

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Cite this paper

@inproceedings{Chen2016Arl2AM, title={Arl2- and Msps-dependent microtubule growth governs asymmetric division}, author={Keng Chen and Chwee Tat Koe and Zhanyuan Benny Xing and X. Y. Tian and Fabrizio Rossi and Cheng Wang and Quan Tang and Wenhui Zong and Wan jin Hong and Reshma Taneja and Fengwei Yu and Cayetano Gonzalez and Chunlai Wu and Sharyn Anne Endow and Hongyan Wang}, booktitle={The Journal of cell biology}, year={2016} }