Argonaute Slicing Is Required for Heterochromatic Silencing and Spreading

@article{Irvine2006ArgonauteSI,
  title={Argonaute Slicing Is Required for Heterochromatic Silencing and Spreading},
  author={Danielle V. Irvine and Mikel Zaratiegui and Niraj H. Tolia and Derek B. Goto and Daniel H. Chitwood and Matthew W. Vaughn and Leemor Joshua-Tor and Robert A. Martienssen},
  journal={Science},
  year={2006},
  volume={313},
  pages={1134 - 1137}
}
Small interfering RNA (siRNA) guides dimethylation of histone H3 lysine-9 (H3K9me2) via the Argonaute and RNA-dependent RNA polymerase complexes, as well as base-pairing with either RNA or DNA. We show that Argonaute requires the conserved aspartate-aspartate-histidine motif for heterochromatic silencing and for ribonuclease H–like cleavage (slicing) of target messages complementary to siRNA. In the fission yeast Schizosaccharomyces pombe, heterochromatic repeats are transcribed by polymerase… Expand
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TLDR
The requirement of slicing for silencing and spreading of histone modifications characteristic of heterochromatin in Schizosaccharomyces pombe is reviewed. Expand
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TLDR
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TLDR
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TLDR
Current understanding of how RITS promotes heterochromatin formation and how it participates in transcription‐coupled silencing is reviewed. Expand
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TLDR
It is shown that slicer activity is required for the siRNA-dependent association of Ago1 with chromatin and for the spreading of histone H3-K9 methylation. Expand
An RNA Degradation Complex Required for Spreading and Epigenetic Inheritance of Heterochromatin
TLDR
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Argonaute: A scaffold for the function of short regulatory RNAs.
TLDR
Recently, the members of the Piwi sub-family have been shown to interact with new classes of short regulatory RNAs, Piwi-interacting RNAs (piRNAs) and repeat-associated small interferingRNAs (rasiRNAs), which has implications for developmental processes and introduces a new dimension to the field of RNA silencing. Expand
Targeting Argonaute to chromatin.
TLDR
Experiments are conducted that address the possibility that siRNAs base-pair directly with DNA, yielding additional evidence that fission yeast siRNA-Argonaute silencing complexes are recruited to target loci exclusively via interactions with nascent transcripts. Expand
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