Argininosuccinate synthetase activity in cultured human lymphocytes

  title={Argininosuccinate synthetase activity in cultured human lymphocytes},
  author={Oksana Lockridge and Elaine Spector and Arthur D. Bloom},
  journal={Biochemical Genetics},
The activity of argininosuccinate synthetase (E.C., a urea cycle enzyme, was measured in cultured human lymphocytes using a new radioactive assay. Control cells had a maximum specific activity of 15.7±8.7 nmoles per hour per milligram of protein and an apparent K m for citrulline of 2 × 10−4 m, whereas cells derived from a patient with citrullinemia had no detectable activity. A nutritional variant, selected out of the citrullinemic lymphocyte population by ability to grow in… CONTINUE READING

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[Colorimetric determination of urinary urea with nioxime].

Clinica chimica acta; international journal of clinical chemistry • 1967

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