Arginine-grafted bioreducible poly(disulfide amine) for gene delivery systems.

Abstract

Arginine-grafted bioreducible poly(disulfide amine) (ABP) polymer was synthesized for non-viral gene delivery systems. Its Mw was measured to be 4.45x10(3) Da/mole by FPLC-SEC and its PDI value was 1.49. ABP was able to retard pDNA from a weight ratio of 2 but ABP could not retard pDNA even at a weight ratio of 10 in the presence of DTT, showing that it can be biodegraded in reducing environment such as cytoplasm. ABP was examined to form positively charged nano-sized particles (<200 nm) with pDNA. ABP showed no significant cytotoxicity and greatly enhanced transfection efficiency in comparison with unmodified poly(cystaminebisacrylamide-diaminohexane) (poly(CBA-DAH)) and PEI25k in mammalian cells. The transfection efficiency of ABP was not much reduced even in the serum condition. Chloroquine treatment was not found to improve the transfection efficiency of ABP. The cellular uptake pattern of ABP polyplexes was almost similar with poly(CBA-DAH), suggesting that greatly enhanced transfection efficiency of ABP is not induced by its high cellular penetrating ability but may be mediated by other factors such as good nuclear localization ability.

DOI: 10.1016/j.biomaterials.2008.10.009

8 Figures and Tables

0502009201020112012201320142015201620172018
Citations per Year

276 Citations

Semantic Scholar estimates that this publication has 276 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Kim2009ArgininegraftedBP, title={Arginine-grafted bioreducible poly(disulfide amine) for gene delivery systems.}, author={Tae-Il Kim and Mei Ou and Minhyung Lee and Sung Wan Kim}, journal={Biomaterials}, year={2009}, volume={30 4}, pages={658-64} }