Arginine degradation by arginase in mitochondria of soybean seedling cotyledons

  title={Arginine degradation by arginase in mitochondria of soybean seedling cotyledons},
  author={Ariel Goldraij and Joseph C. Polacco},
 Arginase (EC localization was studied in soybean (Glycine max L.) seedling cotyledons. Subcellular fractionation in a discontinuous Percoll gradient showed that arginase was localized in the mitochondrion. Arginine (Arg) uptake by mitochondria was demonstrated by co-sedimentation of [3H]Arg-derived label and the mitochondrial marker enzyme cytochrome c oxidase. Arginine uptake was complete in about 10 min. Since detergent but not NaCl released most label, we conclude that Arg was… CONTINUE READING
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