Arginine decarboxylase from Lathyrus sativus seedlings. Purification and properites.

@article{Ramakrishna1975ArginineDF,
  title={Arginine decarboxylase from Lathyrus sativus seedlings. Purification and properites.},
  author={Sridhar Ramakrishna and P. Radhakantha Adiga},
  journal={European journal of biochemistry},
  year={1975},
  volume={59 2},
  pages={377-86}
}
Arginine decarboxylase which makes its appearance in Lathyrus sativus seedlings after 24 h of seed germination reaches its highest level around 5-7 days, the cotyledons containing about 60% of the total activity in the seedlings at day 5. The cytosol enzyme was purified 977-fold from whole seedlings by steps involving manganese chloride treatment, ammonium sulphate and acetone fractionations, positive adsorption on alumina C-gamma gel, DEAE-Sephadex chromatography followed by preparative disc… CONTINUE READING

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