Arginine as an eluent overcomes the hindrance of monoclonal antibody quantification by dextran sulfate in protein A affinity chromatography.

@article{Kim2015ArginineAA,
  title={Arginine as an eluent overcomes the hindrance of monoclonal antibody quantification by dextran sulfate in protein A affinity chromatography.},
  author={Bong Gyun Kim and Hong Woo Park},
  journal={Biotechnology progress},
  year={2015},
  volume={31 6},
  pages={1536-41}
}
Analytical chromatography using protein A affinity columns was employed for the fast and simple quantitative analysis of monoclonal antibodies (mAb) from suspension cultures of recombinant Chinese hamster ovary (rCHO) cells. Reliable results could not be obtained from analysis of rCHO cell culture supernatants containing dextran sulfate using elution… CONTINUE READING