Areca-nut toxicity in cultured human buccal epithelial cells.

  title={Areca-nut toxicity in cultured human buccal epithelial cells.},
  author={Kerstin Sundqvist and Yang Liu and Peter Erhardt and J. Hariharan Nair and Helmut Bartsch and Roland C Grafstr{\"o}m},
  journal={IARC scientific publications},
In cultured human buccal epithelial cells, at doses of 3-540 micrograms/ml, areca-nut extract significantly decreased viability, as determined by colony-forming efficiency, clonal growth rate, ability to take up neutral red and ability to exclude trypan blue, and also caused significant formation of DNA single-strand breaks and DNA protein cross-links. Comparisons of the areca nut-related compounds, 3-(N-nitrosomethylamino)propion-aldehyde (NMPA), 3-(N-nitrosomethylamino)propionitrile (NMPN), N… CONTINUE READING