Architecture of the Protein-Conducting Channel Associated with the Translating 80S Ribosome

@article{Beckmann2001ArchitectureOT,
  title={Architecture of the Protein-Conducting Channel Associated with the Translating 80S Ribosome},
  author={Roland Beckmann and Christian Spahn and Narayanan Eswar and J{\"u}rgen Helmers and Pawel A. Penczek and Andrej Sali and Joachim Frank and G{\"u}nter Blobel},
  journal={Cell},
  year={2001},
  volume={107},
  pages={361-372}
}
In vitro assembled yeast ribosome-nascent chain complexes (RNCs) containing a signal sequence in the nascent chain were immunopurified and reconstituted with the purified protein-conducting channel (PCC) of yeast endoplasmic reticulum, the Sec61 complex. A cryo-EM reconstruction of the RNC-Sec61 complex at 15.4 A resolution shows a tRNA in the P site. Distinct rRNA elements and proteins of the large ribosomal subunit form four connections with the PCC across a gap of about 10-20 A. Binding of… CONTINUE READING
Highly Influential
This paper has highly influenced 13 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 152 extracted citations

The structure and function of the eukaryotic ribosome.

Cold Spring Harbor perspectives in biology • 2012
View 4 Excerpts
Highly Influenced

Architecture of the ribosome-channel complex derived from native membranes.

Journal of molecular biology • 2005
View 6 Excerpts
Highly Influenced

References

Publications referenced by this paper.
Showing 1-10 of 52 references

A comparison of the ment accessible to aqueous perturbants

J. F. Menetret, M. Radermacher, A. Neuhof, T. A. Rapoport, C. W. Akey
Cell • 2000
View 5 Excerpts
Highly Influenced

Stenkamp, R.E., of the Sec61p complex induced by ligands required for protein

D. Hanein, K. E. Matlack, +10 authors C. W. Akey
Cell • 1996
View 4 Excerpts
Highly Influenced

The signal recognition particle.

View 3 Excerpts
Highly Influenced

The resolution was estimated by the Fourier shell correlation

I. Collinson, C. Breyton, F. Duong, C. Tziatzios, D. Schubert
2001
View 2 Excerpts

proteins L4 and L22 in gating mechanisms of the polypeptide tunnel

I. S. Gabashvili, S. T. Gregory, +5 authors J. Frank
Mol. Cell • 2001
View 2 Excerpts

Brunmicroscopy localization of EF2 in the Saccharomyces cerevisiae

P. Penczek, K. Chakraburtty, +3 authors J. Frank
A resolution • 2000

Similar Papers

Loading similar papers…