Architecture of the Protein-Conducting Channel Associated with the Translating 80S Ribosome

  title={Architecture of the Protein-Conducting Channel Associated with the Translating 80S Ribosome},
  author={Roland Beckmann and Christian Spahn and Narayanan Eswar and J{\"u}rgen Helmers and Pawel A. Penczek and Andrej Sali and Joachim Frank and G{\"u}nter Blobel},
In vitro assembled yeast ribosome-nascent chain complexes (RNCs) containing a signal sequence in the nascent chain were immunopurified and reconstituted with the purified protein-conducting channel (PCC) of yeast endoplasmic reticulum, the Sec61 complex. A cryo-EM reconstruction of the RNC-Sec61 complex at 15.4 A resolution shows a tRNA in the P site. Distinct rRNA elements and proteins of the large ribosomal subunit form four connections with the PCC across a gap of about 10-20 A. Binding of… CONTINUE READING
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