Architecture of recombination intermediates visualized by in-gel FRET of lambda integrase-Holliday junction-arm DNA complexes.

@article{RadmanLivaja2005ArchitectureOR,
  title={Architecture of recombination intermediates visualized by in-gel FRET of lambda integrase-Holliday junction-arm DNA complexes.},
  author={Marta Radman-Livaja and Tapan Biswas and Dale F. Mierke and Arthur Landy},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 11},
  pages={3913-20}
}
Lambda integrase (Int) mediates recombination between attachment sites on phage and Escherichia coli DNA. Int is assisted by accessory protein-induced DNA loops in bridging pairs of distinct "arm-type" and "core-type" DNA sites to form synapsed recombination complexes that subsequently recombine by means of a Holliday junction (HJ) intermediate. An in-gel FRET assay was developed and used to measure 15 distances between six points in two Int-HJ complexes containing arm-DNA oligonucleotides, and… CONTINUE READING

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