Architecture of implanted bone matrix gelatin influences heterotopic calcification and new bone formation.

@article{Yamashita1991ArchitectureOI,
  title={Architecture of implanted bone matrix gelatin influences heterotopic calcification and new bone formation.},
  author={Kohei Yamashita and Y Horisaka and Yoshimi Okamoto and Yasunori Yoshimura and Naoki Matsumoto and Juri Kawada and Toshiyuki Takagi},
  journal={Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine},
  year={1991},
  volume={197 3},
  pages={342-7}
}
Heterotopic bone formation in skeletal muscle induced by compacted demineralized bone matrix gelatin (BMG) was studied histologically and biochemically. BMG was obtained by dehydrating diaphyseal shafts of femora and tibiae of 4-week-old male Sprague-Dawley rats, cutting the bone into chips, and demineralizing and extracting the chips with various solutions. The BMG was treated with 4 M guanidine-HCl, and compacted BMG was prepared by centrifugation. The compacted BMG was implanted into the… CONTINUE READING