Archaeal communities of Arctic methane-containing permafrost.

@article{Shcherbakova2016ArchaealCO,
  title={Archaeal communities of Arctic methane-containing permafrost.},
  author={Victoria A. Shcherbakova and Yoshitaka Yoshimura and Yana V. Ryzhmanova and Yukihiro Taguchi and Takahiro Segawa and Victoria Oshurkova and Elizaveta M. Rivkina},
  journal={FEMS microbiology ecology},
  year={2016},
  volume={92 10}
}
In the present study, we used culture-independent methods to investigate the diversity of methanogenic archaea and their distribution in five permafrost samples collected from a borehole in the Kolyma River Lowland (north-east of Russia). Total DNA was extracted from methane-containing permafrost samples of different age and amplified by PCR. The resulting DNA fragments were cloned. Phylogenetic analysis of the sequences showed the presence of archaea in all studied samples; 60%-95% of… CONTINUE READING
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