Arachidonic and eicosapentaenoic acid metabolism by human CYP1A1: highly stereoselective formation of 17(R),18(S)-epoxyeicosatetraenoic acid.

@article{Schwarz2004ArachidonicAE,
  title={Arachidonic and eicosapentaenoic acid metabolism by human CYP1A1: highly stereoselective formation of 17(R),18(S)-epoxyeicosatetraenoic acid.},
  author={Dieter Schwarz and Pyotr A Kisselev and Spencer S. Ericksen and Grazyna D. Szklarz and Alexey Chernogolov and H. C. Honeck and W. -H. Schunck and Ivar Roots},
  journal={Biochemical pharmacology},
  year={2004},
  volume={67 8},
  pages={1445-57}
}
Human cytochrome P450 1A1 (CYP1A1) and human NADPH-cytochrome P450 reductase were expressed and purified from Spodoptera frugiperda insect cells. A reconstituted enzymatically active system metabolized polyunsaturated fatty acids such as arachidonic (AA) and eicosapentaenoic acid (EPA). CYP1A1 was an AA hydroxylase which oxidizes this substrate at a rate of 650+/-10 pmol/min/nmol CYP1A1, with over 90% of metabolites accounted for by hydroxylation products and with 19-OH-AA as major product… CONTINUE READING

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