Arachidonic acid release in cell lines transfected with muscarinic receptors: a simple functional assay to determine response of agonists.

@article{Bymaster1999ArachidonicAR,
  title={Arachidonic acid release in cell lines transfected with muscarinic receptors: a simple functional assay to determine response of agonists.},
  author={F. Bymaster and D. Calligaro and J. Falcone},
  journal={Cellular signalling},
  year={1999},
  volume={11 6},
  pages={
          405-13
        }
}
Muscarinic agonists stimulated arachidonic acid release from 10- to 32-fold in Chinese hamster ovary (CHO) cells transfected with muscarinic M1, M3 and M5 receptor subtypes. Muscarinic agonists liberated arachidonic acid from the cAMP-coupled M2 and M4 cells only in the presence of ATP. Partial agonists were less efficacious at liberating arachidonic acid than full agonists. The ability of muscarinic agonists to liberate arachidonic acid and stimulate phosphoinositide hydrolysis in the same CHO… Expand
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It is concluded that the combined effect of PKC activation and sustained elevation of intracellular calcium, from an extracellular source, is essential for m5 muscarinic receptor activation of phospholipase A2. Expand
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