Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in boars.

  title={Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in boars.},
  author={Kyle W. Lovercamp and Timothy J. Safranski and Kay A Fischer and Gaurishankar Manandhar and Miriam Sutovsky and W. Conyers Herring and Peter Sutovsky},
  volume={67 4},
Accurate semen analysis is an important issue in the swine industry. We evaluated two candidate fertility marker proteins associated with sperm cytoplasmic droplet (CD), including 15-lipoxygenase (15-LOX) and ubiquitin (UBI) in a controlled single-sire artificial insemination (AI) trial. Ejaculates (n=116) were collected from 18 fertile Large White boars monthly for 8 mo, and analyzed by semi-quantitative, densitometry-based Western blotting and flow cytometry with antibodies against 15-LOX and… CONTINUE READING


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