Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis.

@article{Grubb2004ArabidopsisGU,
  title={Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis.},
  author={C. Douglas Grubb and Brandon J Zipp and Jutta Ludwig-M{\"u}ller and Makoto N. Masuno and Tadeusz F Molinski and Steffen Abel},
  journal={The Plant journal : for cell and molecular biology},
  year={2004},
  volume={40 6},
  pages={
          893-908
        }
}
Glucosinolates are a class of secondary metabolites with important roles in plant defense and human nutrition. Here, we characterize a putative UDP-glucose:thiohydroximate S-glucosyltransferase, UGT74B1, to determine its role in the Arabidopsis glucosinolate pathway. Biochemical analyses demonstrate that recombinant UGT74B1 specifically glucosylates the thiohydroximate functional group. Low Km values for phenylacetothiohydroximic acid (approximately 6 microm) and UDP-glucose (approximately 50… CONTINUE READING
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