Arabidopsis acyl-CoA-binding proteins ACBP4 and ACBP5 are subcellularly localized to the cytosol and ACBP4 depletion affects membrane lipid composition

@article{Xiao2008ArabidopsisAP,
  title={Arabidopsis acyl-CoA-binding proteins ACBP4 and ACBP5 are subcellularly localized to the cytosol and ACBP4 depletion affects membrane lipid composition},
  author={Shi Xiao and Hong-Ye Li and Jiao-Ping Zhang and Suk-Wah Chan and M. -L. Chye},
  journal={Plant Molecular Biology},
  year={2008},
  volume={68},
  pages={571-583}
}
In Arabidopsis thaliana, acyl-CoA-binding proteins (ACBPs) are encoded by six genes, and they display varying affinities for acyl-CoA esters. Recombinant ACBP4 and ACBP5 have been shown to bind oleoyl-CoA esters in vitro. In this study, the subcellular localizations of ACBP4 and ACBP5 were determined by biochemical fractionation followed by western blot analyses using anti-ACBP4 and anti-ACBP5 antibodies and immuno-electron microscopy. Confocal microscopy of autofluorescence-tagged ACBP4 and… CONTINUE READING

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Lipid profile analysis further revealed that an acbp4 knockout mutant showed decreases in membrane lipids ( digalactosyldiacylglycerol , monogalactosyldiacylglycerol , phosphatidylcholine , phosphatidylethanolamine and phosphatidylinositol ) while acbp4-complemented lines attained levels similar to wild type , suggesting that ACBP4 plays a role in the biosynthesis of membrane lipids including galactolipids and phospholipids .
Lipid profile analysis further revealed that an acbp4 knockout mutant showed decreases in membrane lipids ( digalactosyldiacylglycerol , monogalactosyldiacylglycerol , phosphatidylcholine , phosphatidylethanolamine and phosphatidylinositol ) while acbp4-complemented lines attained levels similar to wild type , suggesting that ACBP4 plays a role in the biosynthesis of membrane lipids including galactolipids and phospholipids .
Lipid profile analysis further revealed that an acbp4 knockout mutant showed decreases in membrane lipids ( digalactosyldiacylglycerol , monogalactosyldiacylglycerol , phosphatidylcholine , phosphatidylethanolamine and phosphatidylinositol ) while acbp4-complemented lines attained levels similar to wild type , suggesting that ACBP4 plays a role in the biosynthesis of membrane lipids including galactolipids and phospholipids .
Lipid profile analysis further revealed that an acbp4 knockout mutant showed decreases in membrane lipids ( digalactosyldiacylglycerol , monogalactosyldiacylglycerol , phosphatidylcholine , phosphatidylethanolamine and phosphatidylinositol ) while acbp4-complemented lines attained levels similar to wild type , suggesting that ACBP4 plays a role in the biosynthesis of membrane lipids including galactolipids and phospholipids .
Lipid profile analysis further revealed that an acbp4 knockout mutant showed decreases in membrane lipids ( digalactosyldiacylglycerol , monogalactosyldiacylglycerol , phosphatidylcholine , phosphatidylethanolamine and phosphatidylinositol ) while acbp4-complemented lines attained levels similar to wild type , suggesting that ACBP4 plays a role in the biosynthesis of membrane lipids including galactolipids and phospholipids .
Lipid profile analysis further revealed that an acbp4 knockout mutant showed decreases in membrane lipids ( digalactosyldiacylglycerol , monogalactosyldiacylglycerol , phosphatidylcholine , phosphatidylethanolamine and phosphatidylinositol ) while acbp4-complemented lines attained levels similar to wild type , suggesting that ACBP4 plays a role in the biosynthesis of membrane lipids including galactolipids and phospholipids .
Lipid profile analysis further revealed that an acbp4 knockout mutant showed decreases in membrane lipids ( digalactosyldiacylglycerol , monogalactosyldiacylglycerol , phosphatidylcholine , phosphatidylethanolamine and phosphatidylinositol ) while acbp4-complemented lines attained levels similar to wild type , suggesting that ACBP4 plays a role in the biosynthesis of membrane lipids including galactolipids and phospholipids .
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