Arabidopsis TH2 Encodes the Orphan Enzyme Thiamin Monophosphate Phosphatase.

  title={Arabidopsis TH2 Encodes the Orphan Enzyme Thiamin Monophosphate Phosphatase.},
  author={Manaki Mimura and R{\'e}mi G Zallot and Thomas D. Niehaus and Ghulam Hasnain and Satinder K Gidda and Thuy N D Nguyen and Erin M. Anderson and Robert T Mullen and Greg A. Brown and Alexander F Yakunin and Val{\'e}rie de Cr{\'e}cy-Lagard and J. Gregory and Donald R McCarty and Andrew D. Hanson},
  journal={The Plant cell},
  volume={28 10},
To synthesize the cofactor thiamin diphosphate (ThDP), plants must first hydrolyze thiamin monophosphate (ThMP) to thiamin, but dedicated enzymes for this hydrolysis step were unknown and widely doubted to exist. The classical thiamin-requiring th2-1 mutation in Arabidopsis thaliana was shown to reduce ThDP levels by half and to increase ThMP levels 5-fold… CONTINUE READING