Aqueous normal phase chromatography improves quantification and qualification of homocysteine, cysteine and methionine by liquid chromatography-tandem mass spectrometry.

@article{Hellmuth2011AqueousNP,
  title={Aqueous normal phase chromatography improves quantification and qualification of homocysteine, cysteine and methionine by liquid chromatography-tandem mass spectrometry.},
  author={Christian Hellmuth and Berthold Koletzko and Wolfgang Peissner},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2011},
  volume={879 1},
  pages={83-9}
}
Elevation of plasma homocysteine concentration is recognized as an independent predictor of cardiovascular disease risk. Therefore, quantification of homocysteine and related sulphur amino acids cysteine and methionine from plasma samples is routinely performed in clinical laboratories. Due to the highly hydrophilic character of these amino acids, previously reported LC-MS methods often suffered from very short chromatographic retention resulting in inadequate separation from matrix background… CONTINUE READING

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