Aptamers selected for higher-affinity binding are not more specific for the target ligand.

@article{Carothers2006AptamersSF,
  title={Aptamers selected for higher-affinity binding are not more specific for the target ligand.},
  author={James M. Carothers and Stephanie C. Oestreich and Jack W Szostak},
  journal={Journal of the American Chemical Society},
  year={2006},
  volume={128 24},
  pages={7929-37}
}
Previous study of eleven different in vitro-selected RNA aptamers that bind guanosine triphosphate (GTP) with K(d)s ranging from 8 microM to 9 nM showed that more information is required to specify the structures of the higher-affinity aptamers. We are interested in understanding how the more complex aptamers achieve higher affinities for the ligand. In vitro selection produces structural solutions to a functional problem that are are as simple as possible in terms of the information content… CONTINUE READING